Invitrogen公司Qubit Protein Assay Kit

Invitrogen公司Qubit Protein Assay Kit

貨號 單位規(guī)格     單價 (CNY)

Q33211 100 assays   1,468.00

Q33212 500 assays   4,493.00

The Qubit Protein Assay Kit is designed specifically for use with the Qubit Fluorometer. Using between 1 and 20 µl of your sample, this assay can quantitate samples ranging from 12.5 µg⁄ml to 5 mg⁄ml and exhibits low protein to protein variation. The assay is highly selective for proteins and is designed to be accurate in the presence of reducing reagents, but not in the presence of a large amount of detergent. Common contaminants, such as reducing reagents (DTT, β-mercaptoethanol), salts, free nucleotides, amino acids, solvents, or DNA, but not detergents, are well tolerated in the assay. Slight protocol modifications are required for other contaminants. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration.

Which product to choose for fluorometric protein quantitation?

• For 1–20 samples: use this Qubit Protein Assay Kit with the Qubit Fluorometer

• For 20–2000 samples: use the Quant-iT Protein Assay Kit with microplate reader

Notes:

1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.

2. 500 µL thin-walled PCR tubes are required but not included.

規(guī)格

Assay Type: Protein Quantitation Assay

Detection Method: Fluorescent

For Use With (Equipment): Microplate Reader, Qubit® Fluorometer

Number of Reactions: 100 reactions

Product Line: Quant-iT™, Qubit™

Product Size: 100 assays

Quantitation Range: 0.25-5 µg

Shipping Condition: Room Temperature

內(nèi)容及儲存

Store in refrigerator (2–8°C) and protect from light.

Note: 500 µL thin-walled PCR tubes are required but not included.

用于Qubit熒光計的Qubit試劑盒，配合簡單的混合讀取模式，均采用相同的操作方式。其中DNA和RNA分析試劑盒僅需孵育2分鐘，而蛋白分析試劑盒需要15分鐘 。 Qubit 4熒光計自動保存您的數(shù)據(jù)，您可用USB數(shù)據(jù)線或U盤導(dǎo)出。 除了常規(guī)的 dsDNA，寡核苷酸，總RNA，小RNA和蛋白質(zhì)的分析試劑盒，在 Ion Personal Genome Machine(PGM) 測序儀上進行測序之前，可使用專門的Ion Sphere Quality Control Kit在Qubit 4熒光計上進行Ion Sphere顆粒質(zhì)量評估。